CONTROLLED PRINTING OF A CELL SAMPLE FOR KARYOTYPING

摘要

Methods and techniques for controlled printing of a cell sample for karyotyping are provided. The methods can involve matrix printing using on-the-fly printing or dispensing to accurately spread cells within at least one cell sample on a surface in preparation for karyotyping, and further analysis. Advantageously, the methods result in a uniform distribution of chromosomes of the cell suspension or sample on the surface of a substrate which can be substantially discretely identified, and also provide for efficiency in a subsequent staining process and any further analysis of the stained chromosomes using a microscope or other imaging device.

主权项

1. A method of printing a cell sample for karyotyping, comprising:
dispensing droplets from a moving nozzle onto a substrate, said dispensing comprising on-the-fly dispensing of cells on the substrate; and controlling at least one of the speed of the nozzle, the distance between the nozzle and the substrate, and the impact velocity of the droplets onto the substrate such that the cell sample is dispersed uniformly on the substrate with a substantially discrete arrangement of chromosomes of the cell sample to provide for accurate and efficient karyotyping preparation and/or analysis.