METHOD OF ELIMINATING DEPENDENCE OF METHANOL INDUCED PROMOTER ON SINGLE METHANOL CARBON SOURCE

摘要

Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

主权项

1. A method for eliminating the dependence on methanol as the single carbon source of a methanol inducible promoter for driving the expression of an exogenous polypeptide encoding gene, comprising:
(1) providing a methylotrophic yeast, said methylotrophic yeast comprises: expression cassette 1, which expresses an exogenous Mit1 polypeptide; and expression cassette 2, which comprises a methanol inducible promoter and an exogenous polypeptide encoding gene which is operably linked to the promoter; (2) culturing the methylotrophic yeast in (1) under the conditions wherein there is no methanol or methanol is not the only carbon source.