DETECTION SOLUTION AND BIOSENSING METHOD USING SAME

摘要

[Problem] To improve specific binding reaction efficiency and detection accuracy in biosensing using precious metal nanoparticles as a marker. [Solution] This detection solution (1) is a detection solution (1) used for biosensing that detects, by using precious metal nanoparticles (10) as a marker, a substance to be detected (R) consisting of a biological substance and included in a sample to be measured (2), wherein the precious metal nanoparticles (10) are dispersed in a buffer aqueous solution (40), each precious metal nanoparticle having, fixed to the surface (10s) thereof: a probe (20) including a hydrophilic biological substance which specifically binds to the substance to be detected (R); and an amphiphilic SAM (30) having a hydrophobic part (31) and a hydrophilic part (32). The side of the amphiphilic SAM (30) that is fixed to the particle (10) is the hydrophobic part (31). The probe (20) is fixed to the surface (10s) by a chemical bond (Bc).