CELL BASED QUALITY CONTROL BIOASSAYS FOR NUTRICEUTICAL AND MEDICINAL PRODUCTS

摘要

A method for determining the translation initiation inhibitory potency of a composition having an unknown level of translation initiation inhibitory activity which comprises contacting an eI-F2α-WT cell with said composition for a time and at a temperature effective to inhibit proliferation of said cell, measuring the level of inhibition of proliferation of said eIF2α-WT cells induced by said sample and comparing the level of inhibition of proliferation induced by said sample with the level of inhibition of proliferation induced by a standard having a known amount of said activity, the amount of said translation initiation inhibitory activity in said sample being proportional to the level of inhibition of proliferation of said eIF2α-WT cell.

主权项

1. A method for determining the translation initiation inhibitory potency of a composition having an unknown level of translation initiation inhibitory activity which comprises:
contacting an ATCC accession No. PTA-13010 cell with said composition for a time and at a temperature effective to inhibit proliferation of said cell, contacting an ATCC accession No. PTA-13011 cell with said composition for a time and at a temperature effective to inhibit proliferation of said cell, measuring the level of inhibition of proliferation of said ATCC accession No. PTA-13010 cell and said ATCC accession No. PTA-13011 cell induced by said composition, wherein the amount of said translation initiation inhibitory activity in said composition is proportional to the level of inhibition of proliferation of ATCC accession No. PTA-13010 cell, and comparing the level of inhibition of proliferation induced by said composition with the level of inhibition of proliferation induced by a standard having a known amount of said activity, and, identifying the composition as not having translation initiation inhibitory activity if said composition inhibits the proliferation of said ATCC accession No. PTA-13011 cell.