BIOMOLECULE-COMPATIBLE, HIGHLY BRANCHED POLYMER AND BIOMOLECULAR-RECOGNITION SURFACE

摘要

A biomolecule-compatible highly branched polymer obtained by polymerizing a monomer having an alkylene oxide and two or more radically polymerizable double bonds in a molecule in presence of a polymerization initiator in 5 mol % or more and 200 mol % or less relative to the moles of monomer, wherein molecular terminals of biomolecule-compatible highly branched polymer have one biomolecular site of at least one pair from the group of combination pairs of biotin and avidin, an antigen and antibody, polynucleotide and polynucleotide having complementary base sequence thereof, cDNA and mRNA, enzyme and substrate, an enzyme and product, an enzyme and competitive inhibitor, an enzyme (binding site) and coenzyme, an enzyme (binding site) and triazine dye, protease and protease inhibitor, Fc site and protein A, Fc site and protein G, lectin and sugar, hormone receptor and hormone, DNA and DNA binding protein, heparin and fibronectin, and heparin and laminin.

主权项

1. A biomolecule-compatible highly branched polymer obtained by polymerizing a monomer having an alkylene oxide and two or more radically polymerizable double bonds in a molecule in the presence of a polymerization initiator in an amount of 5 mol % or more and 200 mol % or less relative to the number of moles of the monomer, wherein
molecular terminals of the biomolecule-compatible highly branched polymer have one biomolecular site of at least one pair selected from the group consisting of the pairs of combinations of biotin and avidin, an antigen and an antibody, a polynucleotide and a polynucleotide having a complementary base sequence of the former polynucleotide, cDNA and mRNA, an enzyme (an active site) and a substrate, an enzyme (an active site) and a product, an enzyme (an active site) and a competitive inhibitor, an enzyme (a coenzyme binding site) and a coenzyme, an enzyme (a coenzyme binding site) and a triazine dye, protease and a protease inhibitor, a Fc site and protein A, a Fc site and protein G, lectin and a sugar, a hormone receptor and a hormone, DNA and a DNA binding protein, heparin and fibronectin, and heparin and laminin.