发明名称 |
METHOD FOR AMPLIFYING LOCUS IN BACTERIAL CELL |
摘要 |
Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A1-P-M-A2, where A1 and A2 are direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit. |
申请公布号 |
WO2009120929(A8) |
申请公布日期 |
2015.10.22 |
申请号 |
WO2009US38511 |
申请日期 |
2009.03.27 |
申请人 |
DANISCO US INC.;FERRARI, EUGENIO;PERES, CAROLINE |
发明人 |
FERRARI, EUGENIO;PERES, CAROLINE |
分类号 |
C12N15/75;C12P21/02 |
主分类号 |
C12N15/75 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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