发明名称 |
METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION |
摘要 |
A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example. |
申请公布号 |
EP2931911(A1) |
申请公布日期 |
2015.10.21 |
申请号 |
EP20130811709 |
申请日期 |
2013.12.05 |
申请人 |
DANISCO US INC. |
发明人 |
HUA, LING;SCHEFFERS, MARTIJN;VAN BRUSSEL, MARCO;VROEMEN, CASPER;ZHANG, BO;ZHANG, ZHENGHONG |
分类号 |
C12P19/14;A21D8/04;A23L7/104;C11D3/386;C12C11/00;C12N9/28;C12P7/06;D06M16/00 |
主分类号 |
C12P19/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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