| 摘要 |
According to the present invention, a microchip for an integrated rotational polymerase chain reaction (PCR) comprises a unit process unit which is placed to be spaced apart in a radial direction from the center of rotation. The unit process unit includes: a target substance collecting unit for providing a space in which a collecting means is stored, and having an inlet and an outlet, positioned farther than the inlet from the center of the rotation; a specimen storing unit connected to the inlet of the target substance collecting unit, and for providing a space in which a specimen is stored; a cleaning buffer liquid storing unit connected to an inlet of a collecting passage, and for providing a space in which a cleaning buffer liquid is stored; a discharge passage extended to be positioned on the outer side in a radial direction as the distance from an outlet of the collecting passage becomes farther, and including an extension unit extended from the outlet of the collecting passage and a connection unit extended toward one side in a circumferential direction by being bent from an end of the lower side of the extension unit; a first elution buffer liquid storing unit connected to the extension unit, and for providing a space in which a first elution buffer liquid is stored; a second elution buffer liquid storing unit connected to the inlet of the collecting passage, and for providing a space in which a second elution buffer liquid is stored; a waste liquid chamber connected to the connection unit, and positioned on the outer side in the radial direction of the discharge passage; and a target substance chamber connected to the connection unit, and positioned in the outer side in the radial direction of the discharge passage. In addition, a part of the connection unit, connected to the waste liquid chamber, is positioned to face an end of the extension unit, and a part of the connection unit, connected to the target substance chamber, is positioned lower than the part of the connection unit, connected to the waste liquid chamber. |
