Method of double-coating lactic acid bacteria

摘要

The present disclosure relates to production of double-coated lactic acid bacteria using peptides and polysaccharide. Double-coated lactic acid bacteria show improved heat-resistance, acid-resistance, bile-resistance, storage stability and excellent survival rate when reaching intestine.

主权项

1. A method of preparing double-coated lactic acid bacteria, the method comprising:
providing a protein aqueous solution comprising either or both of skim milk and isolated soybean protein as a protein component; adding a proteolytic enzyme to the protein aqueous solution, which causes partial hydrolysis of the protein component in the protein aqueous solution to provide a hydrolyzed product comprising water-soluble peptides and water semi-soluble peptides; cultivating lactic acid bacteria in a fermentation solution comprising the hydrolyzed product, wherein at least part of the water-soluble peptides from the hydrolyzed product are consumed by the lactic acid bacteria, wherein cultivating provides a liquid mixture comprising the lactic acid bacteria, the water-soluble peptides and the water-semisoluble peptides; centrifuging the liquid mixture to deposit the water-semisoluble peptides along with the lactic acid bacteria such that deposited water-semisoluble peptides enclose lactic acid bacteria to provide peptide-coated lactic acid bacteria, collecting, from the centrifugation result, the peptide-coated lactic acid bacteria, mixing, in an aqueous solution, the collected peptide-coated lactic acid bacteria with a polysaccharide and a cryoprotectant to provide a homogenized aqueous mixture comprising the peptide-coated lactic acid bacteria, the polysaccharide and the cryoprotectant, freezing the homogenized aqueous mixture to provide a frozen homogenized mixture in which the peptide-coated lactic acid bacteria are enclosed in a polysaccharide coating, and lyophilizing the frozen homogenized mixture, thereby forming double-coated lactic acid bacteria coated with the water-semisoluble peptides and further with the polysaccharide.