Kit for detecting HIV-1 and method for detecting HIV-1 using the same

摘要

A method is described for the real-time detection of HIV-1 gene target DNA in a sample, including obtaining HIV-1 specific cDNA by reverse transcription, amplifying a portion of the cDNA, and detecting the amplicons so produced using probe labeled with a detectable marker.

主权项

1. A kit for detecting HIV-1, comprising:
a first primer comprising the oligonucleotide of the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, and 8:(SEQ ID NO: 1)GCAGTACAAATGGCAGTATTCATCCACAATT,(SEQ ID NO: 2)CAGCAGTACAAATGGCAGTATTCATCCA,(SEQ ID NO: 3)CAGGAATTCGGGATACCCTACAATCCTCAA,(SEQ ID NO: 4)AGGAATTCGGGATACCCTACAATCCTCAAA,(SEQ ID NO: 5)ACAATCCTCAAAGTCAGGGAGTAGTAGAAT,(SEQ ID NO: 6)CAAAGTCAGGGAGTAGTAGAATCCATGAAT,(SEQ ID NO: 7)CCAAGGGGAAGTGACATAGCAGGAACTACTA,and(SEQ ID NO: 8)CCAAGGGGAAGTGACATAGCAGGAACTACT. a second primer comprising the oligonucleotide of the nucleotide sequence selected from the group consisting of SEQ ID NO: 9, 10, 11, 12, 13, 14, 15, 16, 17, 18 and 19:(SEQ ID NO: 9)CTGACAGGGCTATACATTCTTACTATTTTATT,(SEQ ID NO: 10)TTTCTGCTGTCCCTGTAATAAACCCGAAAATTT,(SEQ ID NO: 11)TCTCTGCTGTCCCTGTAATAAACCCGAAAATTT,(SEQ ID NO: 12)CTCTGCTGTCCCTGTAATAAACCCGAAAATTT,(SEQ ID NO: 13)TCTGCTGTCCCTGTAATAAACCCGAAAATTT,(SEQ ID NO: 14)CTCTGCTGTCTCTGTAATAGACCCGAAAATTT,(SEQ ID NO: 15)CTCTGCTGTCTCTGTAATAGACCCGAAAATT,(SEQ ID NO: 16)CTCTGCTGTCTCTGTAATAGACCCGAAAATTTT,(SEQ ID NO: 17)GTCCTTTCCAAATAGGATCTCTGCTATCTC,(SEQ ID NO: 18)CCAAATAGGATCTCTGCTATCTCTGTAATA,and(SEQ ID NO: 19)AAATAGGATCTCTGCTATCTCTGTAATAGA,and
a probe comprising the oligonucleotide of the nucleotide sequence selected from the group consisting of SEQ ID NO: 20, 21, 22, 23, 24 and 25:(SEQ ID NO: 20)TACCCTTCAGrGrArArCAAATAGGATGGAT,(SEQ ID NO: 21)GGAGAAATTTATAArArArGrATGGATAATCCTG,(SEQ ID NO: 22)TTAAAAGAAAAGGGGrGrGrArUTGGGGGGTACA,(SEQ ID NO: 23)TTAAAAGAAAAGGGGGrGrArUrUGGGGGGTACA,(SEQ ID NO: 24)TGTACCCCCCArArUrCrCCCCCTTTTCTTTTAA,and(SEQ ID NO: 25)TTAAAAGAAAAGG*GG*rGrGrArUTG*GG*GG*GTACA,wherein the nucleotides “rA,” “rG,” “rU, and “rC” are ribonucleotides and the nucleotide G* is 8-Aza-7-Deaza-dG.