Rapid salmonella serotyping assay

摘要

Processes for the serotype specific detection and identification of one or more Salmonella serotypes are provided. A family of specific primers and probes are provided that allow screening of biological or environmental samples for robust, rapid, and reproducible detection and identification of one or more Salmonella serotypes in the sample.

主权项

1. A process of detecting Salmonella in a sample comprising:
performing a first stage assay by adding to a first portion of said sample one or more first stage O-group-1 (O-grp-1) primer sets operable to produce an O-grp-1 amplification product of O-grp-1 target sequence; adding to a second portion of said sample one or more first stage H-antigen (H-ag) primer sets operable to produce an H-ag amplification product of H-ag target sequence; each of said first stage primer sets comprising a forward primer that hybridizes to a region flanking a respective target sequence under conditions suitable for a polymerase chain reaction, and a reverse primer that hybridizes to a different region of the target sequence under conditions suitable for a polymerase chain reaction; and detecting the presence or absence of each of said amplification products by hybridization to a labeled probe specific to said O-grp-1 amplification product or said H-ag amplification product, or both a labeled probe specific to said O-grp-1 amplification product and a labeled probe specific to said H-ag amplification product, wherein said first stage H-ag primer sets comprise primers SEQ ID NOs: 22, 23, 24, 25, 26, 27, 28, 29, 30, 83, 84, 85, 93, 94, 95, 96, 107, 109, 111, 113, 115, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, and 41.