Alkaline-stable chromatographic resins

摘要

This invention is directed to the production of improved chromatographic resins and method of making and using such resins.

主权项

1. A method of preparing an alkaline-stable functionalized solid support which comprises:
(a) contacting a solid support comprising nucleophilic groups which are hydroxyl groups and which may further comprise additional nucleophilic groups which are different than hydroxyl groups, under suitable conditions with an alkaline-stable protected (i) biomonomer or (ii) biopolymer protected with a alkaline-stable protecting group so as to form a coupled solid support; (b) reacting the coupled solid support under suitable conditions so as to cleave any alkaline-labile bonds formed in step (a) between the hydroxyl group and the alkaline-stable protected (i) biomonomer or (ii) biopolymer; (c) acylating the hydroxyl groups in the coupled solid support to form an acylated coupled solid support; (d) removing the alkaline-stable protecting group from the biomonomer or biopolymer to yield a deprotected biomonomer or biopolymer on the acylated coupled solid support; (e) contacting the acylated coupled solid support with suitable reagents so as to link at least one additional protected biomonomer or biopolymer to the deprotected biomonomer or biopolymer on the acylated coupled solid support; and (f) deacylating the acylated coupled solid support so as to form the alkaline-stable functionalized solid support.