RNA labeling method

摘要

A method of sample analysis is provided. In certain embodiments, the method involves: a) obtaining a fragmented RNA sample comprising fragments of long RNA molecules and short RNA molecules; b) ligating an adaptor to an end of the RNA of the fragmented RNA sample to produce an adaptor-ligated sample; c) hybridizing said adaptor-ligated sample to an array of nucleic acid probes; and d) reading said array to obtain an estimate of the abundance of a long RNA in the RNA sample and an estimate of the abundance a small RNA in the RNA sample.

主权项

1. A method of sample analysis comprising:
a) obtaining a fragmented RNA sample comprising:
i. fragments of long RNA molecules, wherein the long RNA molecules are at least 200 nucleotides in length; andii. short RNA molecules of less than 200 nucleotides in length; b) ligating an adaptor to an end of the RNA of said fragmented RNA sample to produce an adaptor-ligated sample that comprises adaptor-ligated RNA molecules comprising: i. adaptor-ligated short RNA comprising an adaptor portion and a short RNA portion; and ii. adaptor-ligated long RNA fragments comprising an adaptor portion and a long RNA portion; c) hybridizing said adaptor-ligated sample to a plurality of nucleic acid probes comprising: i. a first nucleic acid probe that comprises a nucleotide sequence that is complementary to both said adaptor portion and said short RNA portion of said adaptor-ligated short RNA; and ii. a second probe that comprises a nucleotide sequence that is complementary to the long RNA portion of said adaptor-ligated long RNA but not said adaptor portion of said long RNA; and d) analyzing the adaptor-ligated RNA molecules that hybridize to said probes.