Recombinant DNA molecule encoding 5′ UTR capable of preventing inhibition of translation under environmental stresses

摘要

An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:;(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and;(b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.

主权项

1. A recombinant DNA molecule comprising a heterologous promoter operably linked to a nucleotide sequence encoding an mRNA molecule, wherein bases 1 to 7 and bases 12 to 32 from the 5′ end of said mRNA molecule are bases 1 to 7 and 12 to 32 of SEQ ID NO:6, respectively, wherein the nucleotide sequence encoding the mRNA molecule is directly ligated to within 3 nucleotides of transcription initiation point of said promoter.