发明名称 |
Method for culturing stem cells |
摘要 |
In the field of biological technology, a stem cell culture method is provided. The method includes preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and seeding the stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium. The stem cell culture method according to the present invention does not require the treatment of the feeder layer cells to lose the division ability, and is thus simple and safe, thereby effectively solving the problem of contamination caused by animal-derived ingredients in culture of human stem cells at present, greatly reducing the culture cost of the stem cells, and providing a safe, effective, and inexpensive stem cell culture method for the industrialization of the stem cells in the future. |
申请公布号 |
US9163214(B2) |
申请公布日期 |
2015.10.20 |
申请号 |
US201013379651 |
申请日期 |
2010.05.04 |
申请人 |
SHANGHAI ICELL BIOTECHNOLOGY CO., LTD.;SHANGHAI UNITED STEM CELL BIOTECHNOLOGY CO., LTD.;INTERNATIONAL PEACE MATERNITY HOSPITAL |
发明人 |
Lai Dongmei;Cheng Weiwei;Guo Lihe;Liu Tianjin;Jiang Lizhen;Huang Qin |
分类号 |
C12N5/0735;C12N5/074;C12N5/095 |
主分类号 |
C12N5/0735 |
代理机构 |
Perkins Coie LLP |
代理人 |
Perkins Coie LLP |
主权项 |
1. A stem cell culture method, comprising:
a) preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and b) seeding stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium. |
地址 |
Shanghai CN |