Multi-directional sorting with reduced contamination in a flow cytometer

摘要

Disclosed is a collection sled for collecting a waste droplet stream 122 in a waste stream catcher that is located below sample collectors. Contamination of collected samples is reduced by collecting the waste droplet stream at a position that is below the sample collectors. A waste trough is provided that gets progressively larger in a downward direction, which prevents backsplash of waste fluid from the collection cavity. Standard sample collector tubes, as well as 8-well strips and microscope slides can be used with the collection sled to collect sample particles.

主权项

1. A collection sled for a flow cytometer comprising:
a first sample collector located in a first position to collect samples from a first deflected droplet stream in said flow cytometer, said first sample collector having apertures that accept standard size sample collection tubes; a second sample collector located in a second position to collect samples from a second deflected droplet stream in said flow cytometer, said second sample collector having apertures that accept standard size sample collection tubes, said second sample collector spaced apart from said first sample collector to form a trough between said first sample collector and said second sample collector along a side portion of said first sample collector and a side portion of said second sample collector, said trough being aligned with said waste droplet stream so that said waste droplet stream passes through said trough into a cavity formed by said first sample collector and said second sample collector; a waste stream collector formed from said cavity between said first sample collector and said second sample collector that expands in a downward direction from said trough to prevent backsplash of waste fluid from waste droplets in said waste droplet stream and has a waste disposal port for disposing of waste fluid from said waste droplet stream.