Spatial Sequencing of Nucleic Acids Using DNA Origami Probes

摘要

A method of sequencing nucleic acids by probe hybridization and/or ligation is provided using DNA origami as a barcode for a nucleic acid probe.

主权项

1. A method for determining the sequence of nucleotides in a single stranded nucleic acid comprising
imaging the single stranded nucleic acid having an oligonucleotide probe hybridized thereto, wherein the oligonucleotide probe includes a spatially distinct nucleic acid structure corresponding to one or more nucleotides in the oligonucleotide probe, and identifying the spatially distinct nucleic acid structure, the corresponding one or more nucleotides in the oligonucleotide probe and a complementary one or more nucleotides in the single stranded nucleic acid.