NUCLEIC ACID AMPLIFICATION AND USE THEREOF

摘要

The invention features compositions and methods that are useful for the measurement of the quantity of a nucleic acid target in a sample.

主权项

1. A method of amplifying a target nucleic acid molecule in the presence of a nucleic acid probe, the method comprising:
amplifying the target nucleic acid molecule in the presence of a nucleic acid probe, wherein the nucleic acid probe hybridizes to the target nucleic acid molecule and has a probe:template melting temperature (Tm) less than the temperatures used for amplification.