| 发明名称 |
METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE |
| 摘要 |
A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA. |
| 申请公布号 |
US2015291985(A1) |
申请公布日期 |
2015.10.15 |
| 申请号 |
US201314439834 |
申请日期 |
2013.09.20 |
| 申请人 |
SHOWA DENKO K.K. |
发明人 |
Aoki Hirobumi;Kokido Yuzuru;Hashimoto Yoko;Yoneda Tadashi |
| 分类号 |
C12P7/18;C12N9/04 |
主分类号 |
C12P7/18 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof,
wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA. |
| 地址 |
Tokyo JP |
