| 摘要 |
The problems addressed by the inventors were to resolve the drawbacks of the prior art and develop a method for efficiently conducting gene modification in pigs, and to make it possible to breed gene-knockout pigs that die before reaching breeding age. In the present invention, a gene-knockout pig individual was produced by preparing gene-knockout pig cells by cutting a target gene using mRNA that encoded zinc finger nuclease specific to the target gene, removing the cell nuclei, transplanting the cells to an enucleated unfertilized egg, and transferring the egg to the uterus of a surrogate mother. Some of the gene-knockout pigs produced in this way die before they can reach breeding age, similarly to the XSCID pig of the embodiments, but it was also shown that gene-knockout pigs capable of breeding can be produced by blastocyst complementation using a normal embryo. |
