| 摘要 |
<p>THE PRESENT INVENTION RELATES TO A METHOD FOR OBTAINING CAROTENOID STANDARD, COMPRISING THE STEPS OF: GRINDING OENANTHE JAVANICA LEAF SAMPLES INTO FINE POWDER; ADDING AN ACETONE-ALCOHOL MIXTURE TO THE POWDERED LEAF SAMPLES TO EXTRACT A CAROTENOID SOLUTION IN ACETONE, THEN CENTRIFUGING THE CAROTENOID SOLUTION IN ACETONE, THEREBY ISOLATING ACETONE SUPERNATANT FROM FINE PARTICULATES; PARTITIONING THE CAROTENOID SOLUTION IN ACETONE TO A PETROLEUM ETHER, THEREBY OBTAINING A PETROLEUM ETHEREAL EXTRACT CONTAINING THE CAROTENOID; RINSING THE PETROLEUM ETHEREAL EXTRACT WITH DISTILLED WATER TO REMOVE RESIDUAL ACETONE FOR AT LEAST FIVE TIMES; CONCENTRATING THE RINSED PETROLEUM ETHEREAL EXTRACT APPROXIMATELY TEN TIMES; SEPARATING THE CAROTENOID IN THE PETROLEUM ETHEREAL EXTRACT WITH A PLURALITY OF ELUENTS BY OPEN COLUMN CHROMATOGRAPHY; WHEREIN THE PLURALITY OF ELUENTS IS 1% DIETHYL ETHER AND 8% DIETHYL ETHER IN PETROLEUM ETHER; COLLECTING THE BETA-CAROTENE AND WASHING THE BETA-CAROTENE FOR AT LEAST 3 TIMES TO REMOVE ACETONE.</p> |
