MICRO-CHIP FOR DIAGNOSIS AND INTEGRATED ROTARY DIAGNOSIS METHOD USING THE SAME

摘要

Provided is a micro-chip for diagnosis, including a unit process part located apart from a rotation center, which includes: a target substance capturing unit having a capture passage and a capturing means filling a capture passage; a sample storing unit connected to capture passage and giving an inner space in which a sample is stored; a washing buffer chamber connected to the capture passage and giving an inner space in which a washing buffer is stored; an elution buffer chamber connected to the capture passage and giving an inner space in which an elution buffer is stored; a reaction solution chamber giving a space in which a reaction solution required for a PCR process is stored; a discharge passage connected to the target substance capturing unit and the reaction solution chamber; a wasted solution chamber connected to the discharge passage; and a target substance chamber connected to the discharge passage.

主权项

1. A micro-chip for diagnosis, comprising:
a unit process part located apart from a rotation center, wherein the unit process part includes:
a target substance capturing unit having a capture passage with an inlet and an outlet located outside of the inlet in a radial direction, and a capturing means filling the capture passage;a sample storing unit located inside of the target substance capturing unit in a radial direction, connected to the inlet of the capture passage and giving an inner space in which a sample is stored;a washing buffer chamber located inside of the target substance capturing unit in a radial direction, connected to the inlet of the capture passage and giving an inner space in which a washing buffer is stored;an elution buffer chamber located inside of the target substance capturing unit in a radial direction, connected to the inlet of the capture passage and giving an inner space in which an elution buffer is stored;a reaction solution chamber giving a space in which a reaction solution required for a polymerase chain reaction (PCR) process or an isothermal amplification process is stored;a discharge passage located outside of the target substance capturing unit and the reaction solution chamber in a radial direction, extending along a circumferential direction and connected to the target substance capturing unit and the reaction solution chamber;a wasted solution chamber located outside of the discharge passage in a radial direction and connected to the discharge passage; anda target substance chamber located outside of the discharge passage in a radial direction and connected to the discharge passage, wherein a portion of the discharge passage to which the wasted solution chamber is connected and a portion of the discharge passage to which the target substance chamber is connected are separated from each other along a circumferential direction.