METHOD AND DEVICE BASED UPON NOVEL FORMATION OF NUCLEIC ACID DUPLEX ON ARRAY SURFACE

摘要

PROBLEM TO BE SOLVED: To provide: condensed oligonucleotides which are effective in dissociably hybridizing to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from a surface of a device; and a method for identifying a nucleotide sequence to which a nucleotide-binding protein binds using a biomolecular hybridization device and solution-state target nucleic acids capable of hybridizing to probe nucleic acids.SOLUTION: A biomolecular hybridization device comprises a substrate with a semipermanently and covalently attached surface of functional groups, and an adsorbed monolayer of unmodified, single-stranded oligonucleotides which are 10 to about 24 bases in length as a saturated film of compressed oligonucleotides via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide.