CALCIUM-INDEPENDENT SORTASE A MUTANTS

摘要

Disclosed herein are calcium-independent sortase A mutants exhibiting increased catalytic activity compared to wild-type sortase A. In some aspects, the disclosure provides a sortase A mutant comprising at least three amino acid substitutions relative to a wild-type sortase A, wherein the amino acid substitutions comprise a) a K residue at position 105; b) a Q or A residue at position 108; and c) at least one amino acid substitution selected from the group consisting of i) a R residue at position 94; ii) a S residue at position 94; iii) a N residue at position.160; iv) a A residue at position 165; v) a E residue at 5 position 190; and vi) a T residue at position 196. In some embodiments, the mutant comprises a deletion of amino acids 2-25.