Provided herein are various methods for enriching a target fragment that is present in randomly sheared genomic DNA. In some embodiments, the method may involve hybridizing randomly sheared genomic DNA to a halo probe to produce a first circular complex, and then enzymatically digesting the overhanging ends of the genomic fragment. Other embodiments may include hybridizing randomly sheared genomic DNA to an RNA oligonucleotide that comprises a region that hybridizes to a fragment of the randomly sheared genomic DNA to produce an RNA/DNA duplex. The overhanging ends of the genomic fragment in the RNA/DNA duplex can then be enzymatically digested.
申请公布号
EP2929048(A1)
申请公布日期
2015.10.14
申请号
EP20130795318
申请日期
2013.10.03
申请人
AGILENT TECHNOLOGIES, INC.
发明人
HAPPE, SCOTT;LEPROUST, EMILY MARINE;BARBOZA, JULIA;ONG, JOSEPH HOANG HAI