Establishment of patient- or person-specific cardiac myocyte cell lines from human induced pluripotent stem cells (iPSCs)

摘要

A composition and method for generating patient- or person-specific proliferative and substantially pure cardiac myocyte cell lines from pluripotent stem cells (iPSCs) is described herein. The patient-specific cardiac myocyte cell lines of the present invention find applications in research, drug screening and autologous cell-based therapy. The method of the present invention is simple and reproducible and generates cardiac myocyte cells having high purities and proliferating capacities.

主权项

1. A method of obtaining a cardiac cell line from one or more induced pluripotent stem cells (iPSCs) comprising the steps of:
a) culturing iPSCs in culture to form embryoid bodies; b) culturing the embryoid bodies so that they undergo further differentiation into beating and non-beating cardiac cell clusters; c) mechanically separating the beating cell clusters from the non-beating cells; d) culturing the non-beating cells from step c) under conditions wherein some of the cells form into beating cell clusters and some remain non-beating cells; e) mechanically separating the non-beating cells from the beating cell clusters formed in step d); and f) culturing and expanding the non-beating cells from step e) to form a cardiac cell line.