Cartilaginous and osteochondral substitute comprising multilayer structure and use thereof

摘要

The present invention relates to a multilayer structure (1) including a first upper layer (2) consisting of an organic matrix including collagen and at least a lower layer (3, 4, . . . 10) consisting of a composite matrix including hydroxylapatite and collagen. Furthermore, the present invention relates to a cartilaginous substitute including said multilayer structure (1) as well as an osteochondral substitute including said multilayer structure (1). Finally, the present invention relates to the use of said multilayer structure (1) for the preparation of said cartilaginous substitute and said osteochondral substitute for the treatment of cartilaginous defects and osteochondral defects or for the neo-formation of a cartilaginous tissue and/or a subchondral bone tissue.

主权项

1. Cartilaginous or osteochondral substitute comprising a multilayer structure including:
an upper layer consisting of crosslinked collagen fibers; and one or more lower layers consisting of crosslinked collagen fibers and crystals of Mg- and CO3-substituted hydroxylapatite wherein said crystals are nucleated between and parallel to the crosslinked collagen fibers and have dimensions ranging from 10 to 20 nm;wherein said upper layer and said one or more lower layers are in direct contact with each other, and wherein said multilayer structure comprises a collagen-hydroxyapatite gradient characterized by a quantity of crosslinked collagen fibers of 100% in the upper layer and ranging down to between 99% and 1% in at least one lower layer, and wherein the gradient further comprises said crosslinked collagen fibers oriented in a vertical direction.