Method for analyzing sample components

摘要

Described herein is a method and system for on-line coupling of capillary isoelectric focusing (cIEF) to high-resolution mass spectrometry in which a sheath flow buffer comprising polar organic solvent and organic acid is used as both an immobilization solution for (cIEF) and an ionization solution for electrospray ionization (ESI).

主权项

1. A method for identifying and analyzing one or more components in a sample, the method comprising:
a. separating one or more components in the sample by capillary isoelectric focusing (cIEF), wherein the sample and an ampholyte buffer are introduced into a separation chamber and focused under an applied voltage; b. transferring the separated and focused components directly from the separation chamber to an ionization instrument using a sheath flow solution comprising between about 30% and about 50% polar organic solvent and between about 0.01% and 0.1% organic acid; c. ionizing the sample components; and d. identifying and analyzing one or more components in the sample by mass spectrometry (MS).