<i>Cyanobacterium </i>sp. for production of compounds

摘要

A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.

主权项

1. A non-naturally occurring ethanologenic Cyanobacterium sp. organism comprising a genetically enhanced plasmid having a polynucleotide sequence identity of at least 85% to SEQ ID NO: 72 and wherein said genetically enhanced plasmid comprises at least one heterologous alcohol dehydrogenase (adh) gene and at least one heterologous pyruvate decarboxylase (pdc) gene wherein said adh gene encodes an alcohol dehydrogenase that has an amino acid sequence identity of at least 60% to the amino acid sequence of the enzyme encoded by nucleotides 2125 to 3135 of SEQ ID NO: 72 and is operably linked to a promoter and wherein said pdc gene encodes a pyruvate decarboxylase that has an amino acid sequence identity of at least 60% to the amino acid sequence of the enzyme encoded by nucleotides 285-1991 of SEQ ID NO: 72 and is operably linked to a promoter, and wherein said organism produces ethanol from about 0.002% (vol/vol) per day up to about 0.07% (vol/vol) per day.