Methods and combination comprising eukaryotic cells and recombinant spider silk protein

摘要

A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.

主权项

1. A combination, comprising:
eukaryotic cells; and a cell scaffold material; wherein said cell scaffold material comprises a polymer comprising a spider silk protein and a cell-binding motif, said spider silk protein consisting of from 140 to 600 amino acid residues and comprising a repetitive fragment of from 70 to 300 amino acid residues derived from the repetitive fragment of a spider silk protein; a C-terminal fragment of from 70 to 120 amino acid residues derived from the C-terminal fragment of a spider silk protein; and optionally an N-terminal fragment of from 100 to 160 amino acid residues derived from the N-terminal fragment of a spider silk protein, wherein said cell-binding motif is an oligopeptide coupled to the spider silk protein via at least one peptide bond, and wherein said oligopeptide cell-binding motif is a keratinocyte-specific cell-binding motif.