Polymethine compounds and their use as fluorescent labels

摘要

The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

主权项

1. A compound of formula (I) or mesomeric forms thereof: wherein mCat+ or mAn− is an organic or inorganic positively/negatively charged counterion and m is an integer 0-3; each of Ra1 and Ra2 is independently H, SO3−, sulfonamide, halogen, or a further ring fused to an adjacent carbon atom; Rb is optionally substituted aryl or optionally substituted alkyl; each of Rc1 and Rc2 is independently alkyl or substituted alkyl; and either Rb or one of Rc1 or Rc2 contains a linking moiety for further attachment or is linked to a further molecule; wherein one of the Ra groups is a further fused ring forming a structure of formula (II): wherein Ra3 is H, SO3−, sulphonamide or halogen; and Rc1 is alkyl or substituted alkyl.