Agents and methods based on the use of the EDA domain of fibronectin

摘要

This invention relates to the use of a polypeptide in the production of an immunostimulatory agent, said polypeptide comprising a sequence corresponding to the EDA domain of fibronectin, a fragment of the EDA domain which can bind to TLR4 or a variant of said EDA domain or a fragment which can bind to TLR4 and which has a homology of more than 70% with any form or natural fragment of the EDA domain. The invention also relates to the production methods and applications of said agent.

主权项

1. A proteinaceous vector which comprises a polypeptide whose amino acid sequence is selected from:
a) fibronectin EDA domain (EDA), or b) a fragment of an EDA domain capable of binding TLR4, wherein said EDA domain consists of
residues 2 to 91 of SEQ. ID. NO: 2,SEQ. ID. NO: 4,residues 2 to 57 of SEQ. ID. NO: 6, orSEQ. ID. NO: 8 bound to a molecule of interest selected from the group consisting of: a viral antigen, a fungal antigen, a parasitic antigen, a tumoral antigen, and a tumoral antigenic determinant, wherein the proteinaceous vector is capable of translocating the molecule of interest into a TLR4 expressing cell.