Multi-component non-woven

摘要

The formation of a non-woven, free from organic solvent, formed through parallel formation of fibers on a collection device is disclosed. As the individual fibers are dry prior to contact with other fibers, the different contents of the various fiber types do not interact. However, when wetted, the fibers will start to be dissolved, or swell, and the different contents will be released and then interact. For the example of thrombin and fibrinogen, the interaction will initiate the formation of a fibrin coagulum by the cleavage of fibrinogen through the action of thrombin to form fibrin monomers that spontaneously polymerize to form a three dimensional network of fibrin.

主权项

1. A method for preparation of a non-woven comprising the steps of:
(a) ejecting an aqueous alcoholic solution of gelatin comprising thrombin through a nozzle to form gelatin fibers containing thrombin, wherein the aqueous alcoholic solution comprises less than 25% by volume of a low molecular weight alcohol; (b) ejecting an aqueous alcoholic solution of gelatin comprising fibrinogen through a nozzle to form gelatin fibers containing fibrinogen, wherein the aqueous alcoholic solution comprises less than 25% by volume of a low molecular weight alcohol; while (c) emitting pressurized air into the gelatin fibers containing thrombin and the gelatin fibers containing fibrinogen exiting the nozzles to attenuate or stretch the gelatin fibers containing the thrombin and the gelatin fibers containing the fibrinogen; and (d) collecting the gelatin fibers containing the thrombin and the gelatin fibers containing the fibrinogen on a collecting device to form a non-woven multiple-component gelatin scaffold comprising thrombin and fibrinogen.