DETECTION OF SURFACE BOUND MAGNETIC PARTICLES

摘要

The invention relates to a method and a sensor device (100) for the detection of magnetic particles (1) bound to the binding surface (111) of a sample chamber (112) wherein said detection is made during and/or immediately after the action of an attractive magnetic field. Preferably the attractive magnetic field (B) is preceded by a repulsive magnetic field (B) which removes unbound magnetic particles away from the binding surface (111). Due to the attractive magnetic field (B) bound magnetic particles (1) come closer to the binding surface (111) which increases the signal of surface specific detection techniques like frustrated total internal reflection. A further enhancement of the signal can be achieved by an attractive magnetic field that is parallel to the binding surface (111) thus inducing the generation of chains between unbound and bound magnetic particles.