METHOD OF FORMALIN BASED TISSUE FIXATION

摘要

Disclosed are novel cold fixation methods and reagents comprising contacting a biological sample with a reagent for fixation at a temperature of less than 20° C. where the reagent comprises an aqueous buffer, a water soluble alkylnitrile, C2 to C6 alkyl ester, or combination thereof, and formaldehyde to the aqueous buffer solution and removing the biological sample from contact with the reagent.

主权项

1. A method of cold fixation of a biological sample comprising the steps of:
a. contacting the biological sample with a reagent for fixation at a temperature of less than 20° C., the reagent comprising:
an aqueous buffer solution comprising 2-80 volume % of a water soluble alkylnitrile, C2 to C6 alkyl ester, or combination thereof; and0.5 to 20% w/v formaldehyde to the aqueous buffer solution; and b. removing the biological sample from contact with the reagent.