Use of Microparticle Additives to Simultaneously Enable Artifact-Free Image Registration, Auto-Focusing, and Chromatic Aberration Correction in Microscopy

摘要

High-contrast, high-density cell-sized microparticles are introduced into a cell-containing solution prior to the solution being spread onto a planar substrate for imaging. The microparticles facilitate both the process of imager autofocusing and the subsequent registration of multiple images taken of regions of the substrate. The microparticles can further facilitate the correction of chromatic aberration.

主权项

1. A method for using an imager having an auto-focuser to acquire target information from subject targets on a planar substrate, comprising the steps of:
forming a mixture of subject targets and facilitative targets on a planar substrate; in a first focusing step, using the auto-focuser, focusing the imager with respect to facilitative targets within a first region; and in a first imaging step, without refocusing the imager, using the imager to acquire information within the first region from at least subject targets.