SYSTEMS AND METHODS FOR DEEP SPECTROSCOPIC IMAGING OF BIOLOGICAL SAMPLES

摘要

Disclosed herein are systems and methods for deep spectroscopic imaging of a biological sample. In an aspect, a system includes a broad bandwidth light source configured to generate an illumination beam, an interferometer, and a spectrometer. The interferometer includes a first beam splitter configured to split the illumination beam into an incident beam and a reference beam; an optical lens directs the incident beam onto a biological sample at a predefined offset from corresponding optical axis, and receive a beam scattered from the biological sample. The beams are configured to intersect with each other within a focal zone of the optical lens. Photons of the incident beam undergo multiple forward scattering within the biological sample. A second beam splitter configured to receive and superimpose the scattered and reference beams, to generate an interference beam. The spectrometer uses a spectral domain detection technique to assess tissue properties of the biological sample.

主权项

1. A system for deep spectroscopic imaging, the system comprising:
a broad bandwidth light source configured to generate an illumination beam; an interferometer comprising:
a first beam splitter configured to receive and to split the illumination beam into an incident beam and a reference beam;an optical lens configured to direct the incident beam onto a biological sample at a first predefined offset from corresponding optical axis, and receive a beam scattered from the biological sample received at a second predefined offset from the optical axis, wherein the scattered and incident beams are configured to intersect with each other within a focal zone of the optical lens, the focal zone being situated at a predefined distance beneath a surface of the biological sample, wherein one or more photons of the incident beam undergo multiple forward scattering within the biological sample; anda second beam splitter configured to receive and superimpose the scattered and reference beams, to generate an interference beam; and a spectrometer configured to receive the interference beam and to use a spectral domain detection technique to assess one or more tissue properties of the biological sample based on a spectral interference pattern of the interference beam.