METHODS OF IDENTIFYING AN ORGANISM

摘要

This disclosure features methods of identifying an organism. In certain embodiments, the invention provides methods of distinguishing virulent and non-virulent strains of organisms, such as E. coli.

主权项

1. A method of analyzing an unknown strain of an organism, the method comprising:
obtaining a nucleic acid from an unknown strain of an organism; preparing an optical map from the nucleic acid; comparing the optical map of the unknown strain to at least one optical map of a known strain of the organism to identify conserved and non-conserved regions in the optical map of the unknown strain; and
determining the sequence of the non-conserved regions of the unknown strain using primers that hybridize to conserved regions flanking said non-conserved regions.