摘要 |
The invention relates to the field of multi-etiological molecular diagnosis and diagnosis of emerging diseases through the use of DNA arrays. More specifically, the invention relates to oligonucleotides included in an array for the detection of microorganisms that can cause diarrheal disease. Said array contains the oligonucleotides corresponding to Enteroaggregative Escherichia coli, Enterohemorrhagic Escherichia coli, Enterotoxigenic Escherichia coli, Enteropathogenic Escherichia coli, Enteroinvasive Escherichia coli, Giardia intestinalis, Blastocystis hominis, Entamoeba histolytica/dispar, Cryptosporidium parvum, Shigella spp., Uropathogenic Escherichia coli, Salmonella enterica, Vibrio cholerae, among other pathogens that cause gastric diseases in humans or animals. The array consists in immobilising said oligonucleotides in a support once they have been amplified using the PCR technique. The array can be a microarray or a macroarray, and, in one embodiment, it takes the form of a biochip, either a macrochip or microchip. |
申请人 |
UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
发明人 |
SOUZA SALDIVAR, VALERIA FRANCISCA LEOPOLDINA DE MARÍA DE GUADALUPE;EGUIARTE FRUNS, LUIS ENRIQUE;GONZÁLEZ GONZÁLEZ, ANDREA;DELGADO SAPIEN, GABRIELA;MORALES ESPINOSA, MARIA DEL ROSARIO;SANCHEZ REYES, LUNA LUISA;XIMENEZ GARCIA, CECILIA TERESA;CERRITOS FLORES, RENÉ;ZEMEÑO LEÓN, VALERIA;ALCARAZ PERAZA, LUIS DAVID |