METHODS TO REGULATE POLARIZATION AND ENHANCE FUNCTION OF CELLS

摘要

Methods and compositions to controllably regulate cells at a target site. A quantum dot-targeting agent complex is administered to a patient in need of therapy, and the complex is stimulated using an implanted fiber optic system. In embodiments, the system includes an electrical sensor that detects and monitors electrical activity of the stimulated controllably regulated cells, and relays this information to a controller that can regulate further stimulation.

主权项

1. A method for at least one of editing or regulating a gene in a target excitable cell, the method comprising
administering to a patient in need thereof a plurality of nanoparticles coated with a biocompatible molecule for cell uptake, the nanoparticles conjugated with at least one opsin-family gene, an antibody that targets the nanoparticles to an excitable cell, and a CRISPR/cas9 complex forming a complex of coated nanoparticle-gene-CRISPR/cas9, stimulating the complex with an energy source under conditions sufficient to introduce the gene into the target cell and, using the CRISPR/cas9 complex, to modify a pathology-inducing DNA in the cell to treat the patient.