SELECTING FETAL BOVINE SERUM FOR USE IN STEM CELL CULTURE MEDIA AND PREPARING STEM CELL CULTURE

摘要

A method of selecting a collection of cells for choosing a fetal bovine serum (FBS) to use in stem cell transplantation is provided. In addition, a method of selecting a fetal bovine serum (FBS) for use in culturing stem cells for transplantation is provided. Furthermore, a culture medium for culturing stem cells for transplantation and a method of stem cell transplantation are provided.

主权项

1. A method of selecting a fetal bovine serum (FBS) for use in culturing stem cells for transplantation, the method comprising:
providing a plurality of collections of cells; monitoring growth of cells in the plurality of collections; selecting a first one among the plurality of collections at least in part based on the monitoring of cell growth, wherein the first collection falls in inferior half among the plurality of collections when the plurality of collections are ranked in terms of cell growth; providing a plurality of FBS samples from which to select one; formulating a plurality of culture media, each comprising one of the plurality of FBS samples; culturing cells of the first collection in the plurality of culture media; monitoring cell growth in the plurality of culture media; based on monitoring, identifying one or more culture media, among the plurality of culture media, that have shown at least 1.5 fold cell growth after one culturing passage; and selecting at least one FBS that is included in the one or more identified culture media for use in culturing stem cells for transplantation.