发明名称 |
METHOD FOR EVALUATING BOTH INDUCTION OF DRUG-METABOLIZING ENZYME AND CYTOTOXICITY, AND VECTOR AND CELL FOR USE IN SAID METHOD |
摘要 |
It is an object of the present invention to evaluate the induction of a drug-metabolizing enzyme by a test substance more accurately than ever before, by simple operations, using a vector in which a reporter gene is connected downstream of the expression control region of a drug-metabolizing enzyme gene and a cell into which the aforementioned vector is introduced. The present invention provides a vector for producing a cell that is used for evaluation of the induction of a drug-metabolizing enzyme by a test substance and the cytotoxicity of the test substance, the vector comprising an expression control region of a fetus-specific gene, a first reporter gene, an expression control region of a drug-metabolizing enzyme gene, and a second reporter gene, wherein the first reporter gene is located downstream of the expression control region of the fetus-specific gene and the second reporter gene is located downstream of the expression control region of the drug-metabolizing enzyme gene. In addition, the present invention also provides a cell comprising the aforementioned vector and a method for evaluating induction of a drug-metabolizing enzyme by a test substance and cytotoxicity of the test substance using the vector. |
申请公布号 |
US2015284812(A1) |
申请公布日期 |
2015.10.08 |
申请号 |
US201314436229 |
申请日期 |
2013.10.21 |
申请人 |
NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY |
发明人 |
Tada Masako;Oshimura Mitsuo;Kawamura Fumihiko;Tsuji Saori |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A vector for producing a cell that is used for evaluation of induction of a drug-metabolizing enzyme by a test substance and cytotoxicity of the test substance, which comprises an expression control region of a fetus-specific gene, a first reporter gene, an expression control region of a drug-metabolizing enzyme gene, and a second reporter gene,
wherein the first reporter gene is located downstream of the expression control region of the fetus-specific gene, and the second reporter gene is located downstream of the expression control region of the drug-metabolizing enzyme gene. |
地址 |
Tottori JP |