REDUCTION OF ENTROPIC BARRIER OF POLYELECTROLYTE MOLECULES IN A NANOPORE DEVICE WITH AGAROSE GEL

摘要

A mechanism is provided for reducing entropy of a polyelectrolyte before the polyelectrolyte moves through a nanopore. A free-standing membrane has the nanopore formed through the membrane. An agarose gel is formed onto either and/or both sides of the nanopore in the membrane. The agarose gel is a porous material. The polyelectrolyte is uncoiled by driving the polyelectrolyte through the porous material of the agarose gel via an electric field. Driving the polyelectrolyte, having been uncoiled and linearized by the agarose gel, into the nanopore is for sequencing.

主权项

1. A method for reducing entropy of a polyelectrolyte before the polyelectrolyte moves through a nanopore, the method comprising:
providing a free-standing membrane having the nanopore formed through the membrane; forming an agarose gel onto at least one of either or both sides of the nanopore in the membrane, wherein the agarose gel is a porous material; uncoiling the polyelectrolyte by driving the polyelectrolyte through the porous material of the agarose gel via an electric field; and driving the polyelectrolyte, having been uncoiled and linearized by the agarose gel, into the nanopore for sequencing.