A RECOMBINANT PENICILLIN V ACYLASE AND PROCESS FOR THE PREPARTION THEREOF

摘要

The present invention provides recombinant penicillin V acylase represented by SEQ ID No.1 having enhanced activity and increased specificity towards the synthesis of 6-amino-penicillanic acid. The nucleotide sequence encoding the said penicillin V acylase enzyme lacking the periplasmic signal sequence is cloned into a plasmid cloning vector followed by expression of the gene in Escherichia coli to produce the desired recombinant protein i.e. penicillin V acylase in the cytoplasm in increased concentrations.