| 主权项 |
1. An engineered three dimensional pulmonary model tissue culture, wherein said model tissue culture
a) is free of any artificial tissue scaffold, b) comprises cultured pulmonary fibroblasts, c) comprises cultured pulmonary small airway epithelial cells, and d) has a morphology of one or more cellular aggregate(s), each aggregates comprising a multiplicity of cavities, wherein the pulmonary small airway epithelial cells express at least one alveolar type II (ATII) epithelial differentiation marker and wherein the cells are in direct cell-cell contact with cells of one or more other types of the tissue culture, and wherein said pulmonary small airway epithelial cells and pulmonary fibroblasts comprise cultured primary cells obtained from a subject. |
