Rapid detection of mycobacteria

摘要

The invention provides a method for detecting a mycobacterium belonging to the Mycobacterium tuberculosis complex (MTBc) in a sample, the method comprising: (a) contacting the sample with a forward oligonucleotide primer and a reverse oligonucleotide primer; wherein the forward primer hybridizes to a target nucleic acid sequence located within a Mycobacterial Interspersed Repetitive Unit (MIRU) repeat element; and wherein the reverse primer hybridizes to a target nucleic acid sequence located within a MIRU repeat element; (b) extending the forward and reverse primers to generate an amplification product; and (c) detecting the amplification product.

主权项

1. A method for detecting nucleic acid of a mycobacterium belonging to the Mycobacterium tuberculosis (MTBc) in a sample, the method comprising:
(a) contacting the sample with a pair of primers comprising a forward oligonucleotide primer and a reverse oligonucleotide primer, wherein said forward primer hybridizes to a target nucleic acid sequence that is located within multiple Mycobacterial Interspersed Repetitive Unit (MIRU) repeat elements, and wherein said reverse primer hybridizes to a target nucleic acid sequence that is located within the multiple MIRU repeat elements; wherein the forward primer comprises a sequence that is at least 95% identical to a nucleotide sequence selected from SEQ ID NOs: 25-39; and wherein the reverse primer comprises a sequence that is at least 95% identical to the complement of a nucleotide sequence selected from SEQ ID NOs: 40-46; (b) extending said forward and reverse primers to generate at least one amplification product; and (c) detecting the at least one amplification product.