| 摘要 |
The present invention provides articles of manufacture comprising biocompatible nanostructures comprising significantly increased surface area for, e.g., organ, tissue and/or cell growth, e.g., for bone, tooth, kidney or liver growth, and uses thereof, e.g., for in vitro testing of drugs, chemicals or toxins, or as in vivo implants, including their use in making and using artificial tissues and organs, and related, diagnostic, screening, research and development and therapeutic uses, e.g., as drug delivery devices. The present invention provides biocompatible nanostructures with significantly increased surface area, such as with nanotube and nanopore array on the surface of metallic, ceramic, or polymer materials for enhanced cell and bone growth, for in vitro and in vivo testing, cleansing reaction, implants and therapeutics. The present invention provides optically transparent or translucent cell-culturing substrates. The present invention provides biocompatible and cell-growth-enhancing culture substrates comprising elastically compliant protruding nanostructure substrates coated with Ti, TiO2 or related metal and metal oxide films. |
| 主权项 |
1. A product of manufacture comprising:
(a) a biocompatible surface; (b) a plurality of macro or microscale structures fixed or loosely placed on the biocompatible surface, wherein the plurality of macro or microscale structures comprise: (i) a plurality of hairy-shaped or gauze shaped, mesh or mesh screen, wire, woven wire, spring, ribbon, powder, or flake shaped macro or microscale structures, wherein the macro or a microscale structure comprises a Ti, Zr, Hf, Nb, Ta, Mo and/or W metal material, a Ti, Zr, Hf, Nb, Ta, Mo and/or W alloy, a Ti, Zr, Hf, Nb, Ta, Mo and/or W oxide or nitride, and/or stainless steel or ceramic; (ii) a plurality of macro or microscale loose, short-fibers, or flake shaped structures, or macro or microscale particles, or macro or microscale spring-like fibers, made of a material comprising Ti, Zr, Hf, Nb, Ta, Mo and/or W metal material, a Ti, Zr, Hf, Nb, Ta, Mo and/or W alloy, a Ti, Zr, Hf, Nb, Ta, Mo and/or W oxide or nitride, and/or stainless steel or ceramic; (iii) a plurality of macro or microscale micro-wires, micro-fibers or micro-ribbons; (iv) a plurality of macro or microscale spring-like fibers or mesh screen shapes comprising a Ti or metal material, an alloy and/or a ceramic; or (v) any combination of the structures of (i) to (vi); and (c) a plurality of nanostructures on the surfaces of the plurality of macro or microscale structures, wherein the nanostructures comprise:
(i) a biocompatible vertically aligned nanotube array structure;(ii) a lock-in nanostructure comprising a plurality of nanopores or nanotubes,wherein the nanopore or nanotube entrance has a smaller diameter or size than the rest (the interior) of the nanopore or nanotube to exhibit a re-entrant configuration;(iii) a dual structured biomaterial comprising
(A) micro- or macro-pores, wherein the micro or macro pores has an average diameter, or equivalent diameter if the pores are not circular, in the range of between about 0.5-1,000 μm, or between about 1-100 μm; and,(B) a surface area covered with nanotubes;(iv) a plurality of enlarged diameter nanopores and/or nanotubes, wherein the nanopores and/or nanotubes comprise at least 150 nm; or(v) any combination of (i) to (iv) wherein the nanotube structures of any of (c)(i) to (c)(iv) have a thickness of at least about 0.5, or between about 1 and 50 nm, or between about 1 and 100 nm, or between about 10 nm to 500 nm, wherein at least a portion of, or substantially all of, or all of, the surface of the macro or microscale structures of (b) are covered or coated by one or a plurality of nanostructures structures of (c). |
