发明名称 |
Constitutive promoter |
摘要 |
The invention relates to an isolated nucleic acid sequence comprising a promoter, which is a native sequence of Pichia pastoris comprising the nucleic acid sequence of pCS1 of SEQ ID NO:1, or a functionally active variant thereof which is a size variant, a mutant or hybrid of SEQ ID NO:1, or a combination thereof, expression constructs and recombinant host cells comprising the promoter, and a method of producing a protein of interest under the control of the promoter. It further relates to a method to identify a constitutive promoter from eukaryotic cells, and an isolated nucleic acid sequence comprising a promoter which when operatively linked to a nucleotide sequence encoding a protein of interest directs the expression thereof in a host cell at an expression level that is higher than under control of the native pGAP promoter at high and low growth rates. |
申请公布号 |
US9150870(B2) |
申请公布日期 |
2015.10.06 |
申请号 |
US201313835589 |
申请日期 |
2013.03.15 |
申请人 |
Lonza Ltd. |
发明人 |
Mattanovich Diethard;Gasser Brigitte;Prielhofer Roland |
分类号 |
C12N15/81 |
主分类号 |
C12N15/81 |
代理机构 |
Meunier Carlin & Curfman LLC |
代理人 |
Meunier Carlin & Curfman LLC |
主权项 |
1. An isolated nucleic acid sequence comprising SEQ ID NO:1, a variant having at least 90% homology to SEQ ID NO:1, operably linked to a heterologous nucleotide sequence encoding a protein of interest (POI). |
地址 |
Visp CH |