Detecting antigen responsive cells in a sample

摘要

The present invention relates to methods for detecting antigen responsive cells in a sample using multidimensional labeled antigen presenting compounds, such as antigen-major histocompatibility complexes (NHC). Further, the present invention relates to the use of the present multidimensional labeled antigen presenting compounds, such as antigen-major histocompatibility complexes (MHC), for detecting antigen responsive cells in a sample, preferably a single sample, such as a blood sample. The present method allows high-throughput analysis of specific antigen responsive cells, such as T- and B-cells, thereby providing, for example, high-throughput methods for monitoring of diseases or conditions and the development of immunotherapeutics, vaccines, or the identification epitopes or immunogenic amino acid sequences.

主权项

1. A method for detecting antigen responsive cells in a sample comprising:
providing antigen-containing antigen presenting compounds, each antigen-containing antigen presenting compound carrying at least one label, with two or more predetermined antigens, wherein each antigen is represented by at least two different labels; contacting said antigen-containing antigen presenting compounds with said sample; detecting binding of said antigen-containing antigen presenting compounds to said antigen responsive cells, thereby detecting cells responsive to at least one of said antigens; wherein the presence of the at least one of said antigens is detected by detecting a signal emitted by the at least two different labels bound to an antigen responsive cell through said antigen presenting compounds loaded with the at least one of said antigens.