Method for amplifying variation of frequency of signal in piezoelectrical biosensor

摘要

Provided is a method for amplifying a frequency variation of a detected signal in a biosensor that is used for detecting a biomolecule by measuring a change in frequency of an oscillating signal, the change being caused by pressure a biomolecule applies to a piezoelectric substance. The method for amplifying a frequency variation of a detected signal comprises the steps of: (a) applying a sample to a probe being fixed to an upper portion of a substrate of the biosensor to allow a biomolecule in the sample to be bound to the probe; (b) applying protein tagged with a metal particle to the biosensor to allow the protein and the biomolecule to be bound with each other; and (c) applying a metal enhancer to the biosensor to allow the metal enhancer to be bound to the metal particle having been bound to the protein.

主权项

1. A method for amplifying frequency variation of a detected signal in a biomolecule detecting biosensor, the method comprising the steps of:
(a) applying a metal particle to a sample to allow a biomolecule in the sample to be tagged with the metal particle: (b) applying the sample containing the biomolecule tagged with the metal particle to a probe which is fixed to an upper portion of a substrate of the biosensor to allow the biomolecule in the sample to be bound to the probe; and (c) washing the surface of the biosensor with buffer solution which does not contain salt capable of producing a precipitate or which contains low-concentration salt; and (d) applying a metal enhancer to the biosensor to allow the metal enhancer to be bound to the metal particles which is bound to the biomolecule.